Journal: Biomedicines

Article Title: Combined Therapy with Pirfenidone, Metformin, and Mesenchymal Stem Cells Attenuates Bleomycin-Induced Pulmonary Fibrosis in Rats

doi: 10.3390/biomedicines14030642

Figure Lengend Snippet: The levels of apoptotic markers in the lung tissue of the experimental rats. ( A ) Bcl-2 and ( B ) BAX. Bcl-2 = B-cell lymphoma-2; BAX = Bcl-2-associated X protein; PFD = pirfenidone; MET = metformin; BM-MSCs = bone marrow-derived mesenchymal stem cells. The parameters were detected by a colorimetric method. Data are expressed as mean ± SD. Data were analyzed by one-way ANOVA followed by Bonferroni’s post hoc test. Differences were considered significant at p < 0.05 ( n = 8). a significantly different vs. normal control, b significantly different vs. BLM-positive control, c significantly different vs. PFD alone, d significantly different vs. MET alone, e significantly different vs. BM-MSC group.

Article Snippet: Markers related to apoptosis, BAX and Bcl-2, were measured using the Rat Apoptosis Regulator (BAX) ELISA Kit and the Rat B-cell CLL/Lymphoma 2 (Bcl-2) ELISA Kit (Cusabio, USA, Cat No. CSB-EL002573RA and CSB-E08854r, respectively).

Techniques: Derivative Assay, Control, Positive Control

Journal: Antioxidants

Article Title: Nano-Cilostazol Mitigates Cisplatin-Induced Nephrotoxicity in Rats via Modulation of Oxidative Stress, Apoptosis, Pyroptosis, and miRNA-155 Signaling

doi: 10.3390/antiox15030315

Figure Lengend Snippet: Effects of Nano-Cilostazol on renal ( A ) nuclear factor kappa B (NF-κB) immunoexpression in different groups (Scale bar = 50 µm). ( B ) Area % of NF-κB immunostaining. ( C ) TNF-α (Tumor necrosis factor alpha, pg/mg protein). ( D ) Il-1β (Interleukin-1β, pg/mg protein). ( E ) CRP (C-reactive protein, ng/mL). ( F ) NGAL (Neutrophil Gelatinase-associated Lipocalin, pg/mL). ( G ) Caspase-3 immunoexpression in different groups (Scale bar = 50 µm).( H ) Area% of caspase-3 immunoreactivity. ( I ) BCL2 (B-cell lymphoma 2, ng/mg). ( J ) BAX (BCL-2-associated X protein, ng/mg) and ( K ) BAX/BCL2 ratio. CIS = Cisplatin. Arrows indicate positive nuclear brown immunostaining for NF-κB and caspase-3. Bars bearing different letters were substantially different at p < 0.05. Results are presented as means with SE.

Article Snippet: Rat ELISA kits for BAX, Bcl2, and Caspase-1 (EL002573RA, CSB-E08854r, and CSB-EL004543RA, respectively) were purchased from CUSABIO Co. (Houston, TX, USA).

Techniques: Immunostaining